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New polymorphic microsatellite loci for population studies in the European anchovy, Engraulis encrasicolus (L.)
V Pakaki1, A Magoulas, P Kasapidis
1Institute of Marine Biology and Genetics, Hellenic Centre for Marine Research, PO Box 2214, 71003 Heraklion, Greece.
Abstract:
Eleven microsatellite loci were developed in the European anchovy, Engraulis encrasicolus, and tested in samples from two geographically distant populations (Atlantic and Mediterranean Sea). Number of alleles ranged from eight to 28 and observed heterozygosity from 0.440 to 0.920. There was no evidence of linkage disequilibrium, although two loci are indeed linked. All loci were in Hardy-Weinberg equilibrium, except for one locus in the Atlantic and two loci in the Mediterranean sample. These three loci plus two more showed evidence for null alleles.
