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Updated: Jun 2, 2026
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Development of 11 polymorphic microsatellite markers in a macrophyte of conservation concern, Vallisneria americana
Robert K Burnett1, Michael W Lloyd, Katharina A M Engelhardt
1Department of Plant Science and Landscape Architecture and Department of Entomology, University of Maryland-College Park, 2116 Plant Sciences Building, College Park, MD 20742-4452, USA Appalachian Laboratory, University of Maryland Center for Environmental Science, 301 Braddock Road, Frostburg, MD 21532-2307, USA.
Abstract:
Vallisneria americana Michaux (wild celery) is currently a target of submersed aquatic vegetation restoration efforts in the Chesapeake Bay watershed. To aid these efforts, we have developed 11 polymorphic microsatellite markers to assess the distribution and degree of genetic diversity in both restored and naturally occurring populations in the Chesapeake Bay. In 59 individuals from two populations, we detected two to 10 total alleles per locus. Observed heterozygosity ranged from 0.125 to 0.929, and two loci exhibited significant deviations from Hardy-Weinberg equilibrium in at least one of the populations assayed.
