Related Experiment Videos
Effects of DNA-binding drugs on T4 DNA ligase
A Montecucco1, G Pedrali-Noy, S Spadari
1Istituto di Genetica Biochimica ed Evoluzionistica, CNR, Pavia, Italy.
The Biochemical Journal
|March 1, 1990
Abstract:
A number of DNA intercalating and externally binding drugs have been found to inhibit nick sealing, cohesive and blunt end ligation, AMP-dependent DNA topoisomerization and EDTA-induced DNA nicking mediated by bacteriophage T4 DNA ligase. The inhibition seems to arise from drug-substrate interaction so that formation of active DNA-Mg2(+)-AMP-enzyme complex is impaired while assembled and active complexes are not disturbed by drug binding to the substrate.