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Inactivation of aphidicolin by plant cells.
1Istituto di Genetica Biochimica ed Evoluzionistica, C.N.R., Via S. Epifanio 14, Pavia, Italia.
Plant Cell Reports
|November 22, 2013
Summary
Plant cells possess aphidicolin inactivating activity, with Daucus carota cells showing the highest effectiveness. This inactivation impacts experiments requiring prolonged aphidicolin exposure, necessitating careful condition selection.
Area of Science:
- Plant Cell Biology
- Biochemistry
Background:
- Aphidicolin is a DNA polymerase inhibitor used in cell cycle studies.
- Understanding drug inactivation is crucial for experimental design.
Purpose of the Study:
- To investigate the aphidicolin inactivating activity in various plant cells.
- To identify plant species with significant aphidicolin inactivation capabilities.
Main Methods:
- Testing aphidicolin inactivation in cultured plant cells (Daucus carota, Oryza sativa, Nicotiana plumbaginifolia).
- Assessing inactivation in germinating seedlings (Haplopappus gracilis, Citrullus vulgaris).
Main Results:
- Plant cells exhibit varying rates of aphidicolin inactivation.
- Daucus carota cells demonstrated the highest inactivation rate.
- Germinating seedlings of Haplopappus gracilis and Citrullus vulgaris also inactivated aphidicolin.
Conclusions:
- Plant cells possess inherent aphidicolin inactivating activity.
- The rate of inactivation is cell-type dependent.
- Experimental conditions must be optimized to account for this inactivation, especially in prolonged aphidicolin treatments like cell cycle synchrony induction.
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