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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Related Experiment Video

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A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

Biosensor for direct cell detection, quantification and analysis.

Sandra Cortès1, Christian L Villiers, Pascal Colpo

  • 1INSERM, U823, Grenoble, F-38042, France.

Biosensors & Bioelectronics
|May 14, 2011
PubMed
Summary
This summary is machine-generated.

This study enhances antibody microarrays with surface plasmon resonance imaging (SPRi) for sensitive cell detection. The optimized method accurately identifies cell surface markers and detects rare cells in complex samples without harming them.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Analytical Chemistry

Background:

  • Microarrays show potential for cell isolation and detection but face challenges in sensitivity and complex sample compatibility.
  • Demonstrating sensitivity and lack of aberrant cellular effects is crucial for widespread biological application.

Purpose of the Study:

  • To characterize and optimize antibody microarrays coupled with surface plasmon resonance imaging (SPRi).
  • To validate the system's sensitivity, specificity, and compatibility with complex biological samples.
  • To assess the impact of antibody-antigen interactions on cell morphology and viability.

Main Methods:

  • Utilized antibody microarrays integrated with SPRi for cell detection.
  • Employed a murine macrophage cell line for characterization.
  • Applied confocal and fluorescent microscopy to analyze cell morphology and viability.
  • Optimized the system by applying a polyethylene oxide film to the chip surface.

Main Results:

  • Demonstrated specific binding of antibody microarrays to target cells.
  • Correlated SPRi signals with cell number and cell surface marker expression levels.
  • Showed that cell binding induced morphological changes without affecting cell viability.
  • Achieved a 10-fold lower detection threshold, enabling detection of cells at 0.5% in mixed populations.

Conclusions:

  • The optimized antibody microarray-SPRi system offers high sensitivity and specificity for cell detection.
  • The method is suitable for analyzing complex biological samples, including peripheral blood mononuclear cells.
  • This technology provides a robust tool for characterizing ligand-cell receptor interactions and determining receptor specificity.