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Updated: Jun 2, 2026

Preparation of Mechanically Stable Self-Assembled Peptides Hydrogels
Published on: September 6, 2024
Dependence of Self-Assembled Peptide Hydrogel Network Structure on Local Fibril Nanostructure
Rohan A Hule1, Radhika P Nagarkar, Boualem Hammouda
1Department of Materials Science and Engineering and Delaware Biotechnology Institute, University of Delaware, Newark, Delaware 19716.
Abstract:
Physically cross-linked, fibrillar hydrogel networks are formed by the self-assembly of β-hairpin peptide molecules with varying degrees of strand asymmetry. The peptide registry in the self-assembled state can be used as a design element to generate fibrils with twisting, nontwisting, or laminated morphology. The mass density of the networks varies significantly, and can be directly related to the local fibrillar morphology as evidenced by small angle neutron scattering (SANS) and in situ substantiation using cryogenic transmission electron microscopy (cryo-TEM) under identical concentrations and conditions. Similarly, the density of the network is dependent on changes in the peptide concentration. Bulk rheological properties of the hydrogels can be correlated to the fibrillar nanostructure, with the stiffer, laminated fibrils forming networks with a higher G' as compared to the flexible, singular fibrillar networks.
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