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Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
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Versatile immunoassays based on isomagnetophoresis

Young Ki Hahn1, Je-Kyun Park

  • 1Department of Bio and Brain Engineering, College of Life Science and Bioengineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 305-701, Republic of Korea.

Lab on a Chip
|May 14, 2011
PubMed

We report an isomagnetophoretic immunoassay capable of detecting an attomolar level of proteins and adjusting the dynamic range of target analytes. Here, the magnetic nanoparticles are used as labels on microbeads in sandwich-type immunoassay, detecting the amount of bound analytes by isomagnetophoretic focusing the solid-support microbeads under the magnetic susceptibility gradient and magnetic field in a microchannel. For the practical purpose, this platform was applied to detect three types of breast cancer biomarkers.

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