Related Experiment Video
Updated: Jun 2, 2026
Measuring Cell Cycle Progression Kinetics with Metabolic Labeling and Flow Cytometry
Published on: May 22, 2012
Cell-cycle kinetics of permanent glioma-cells measured by pulse labeling with bromodeoxyuridine
1BRIGHAM & WOMENS HOSP,DEPT PATHOL,BOSTON,MA 02115. NEW ENGLAND DEACONESS HOSP,CANC RES INST,BOSTON,MA 02215. HARVARD UNIV,SCH MED,BOSTON,MA 02115. UNIV HAMBURG,DEPT NEUROPATHOL,W-2000 HAMBURG 13,GERMANY.
Abstract:
Cell phase distribution and cycle kinetics of six human glioblastoma cell lines were characterized after labelling with 5-Bromo-2-deoxyuridine (BrdUrd). Cycle time (T(c)), DNA synthesis time (T(s)), and potential doubling time (T(pot)) were compared with the actual doubling time (T(d)) of the growing cell population. Mathematical estimates closely correlated with T(d). Low labelling index (LI) correlated with short T(s) and vice versa. T(s) and LI allowed grouping of the cell lines in two clusters. The mean number of silver stained nucleolar organizer regions (mAgNORs) and percentage of cells with more than five AgNORs (pAgNOR) were counted. AgNORs closely related to LI. Low mAgNORs and pAgNORs correlated with fast T(s) among the clustered cell lines.
