Related Experiment Video
Updated: Jun 2, 2026
07:44
TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
Published on: June 8, 2020
Proteomic profiling of k-11706 responsive proteins
1Graduate School of Comprehensive Human Sciences, University of Tsukuba, Department of Sports Medicine, Tsukuba, Japan.
International Journal of Sports Medicine
|May 14, 2011
Summary
Novel GATA inhibitors, used to treat anemia, could be misused in sports like erythropoietin. Proteomic analysis identified fetuin-B as a potential biomarker for detecting GATA inhibitor abuse in athletes.
Area of Science:
- Biochemistry
- Pharmacology
- Sports Science
Background:
- Erythropoietin (EPO) enhances red blood cell production and is illicitly used in sports.
- GATA-binding protein (GATA) transcription factors regulate EPO gene expression.
- GATA inhibitors are emerging drugs for anemia treatment and potential performance-enhancing agents.
Purpose of the Study:
- To investigate plasma protein alterations following GATA inhibitor administration.
- To identify potential biomarkers for detecting the illicit use of GATA inhibitors in sports.
Main Methods:
- Proteomic analysis of mouse plasma samples treated with the GATA inhibitor K-11706.
- Gel electrophoresis to identify differentially expressed protein spots.
- Liquid chromatography-tandem mass spectrometry (LC-MS/MS) and Western blotting for protein identification and verification.
Main Results:
- Proteomic analysis revealed 41 differentially expressed protein spots in plasma after K-11706 treatment.
- Fetuin-B and prothrombin were identified as significantly altered proteins.
- K-11706 administration increased plasma fetuin-B levels, unlike recombinant human EPO or hypoxia.
Conclusions:
- Proteomic approaches can identify biomarkers for detecting the abuse of novel drugs like GATA inhibitors.
- Fetuin-B shows promise as a sensitive biomarker for detecting GATA inhibitor misuse in sports doping.
Related Concept Videos
01:33Proteomics
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
02:24Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
02:26Protein Networks
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...