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Published on: September 1, 2015
Human cd4+ cd45ra+ lymphocytes-T can be stimulated by crocidolite, anthophyllite and amosite asbestos invitro
Y Watanabe1, M Yamaguchi, Y Kawakami
1KAWASAKI MED UNIV,DEPT HYG,MATSUSHIMA 577,KURASHIKI,OKAYAMA 70101,JAPAN. KAWASAKI MED UNIV,DEPT MED,KURASHIKI,OKAYAMA 70101,JAPAN.
Abstract:
Crocidolite, anthophyllite and amosite asbestos stimulate T lymphocyte subsets. Cell surface CD4 or CD45RA expression in peripheral blood mononuclear cells (PBMC) was downregulated temporarily after incubation with asbestos in vitro. The percentage of CD4+ CD45RA+ cells significantly decreased 12 h after incubation with asbestos, suggesting activation of the cells. An early increase in the intracellular Ca++ level was also indicative of activation. An elevated Ca++ level was observed after incubation in both PBMC and purified T cell fractions.
