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Development and characterization of 60 microsatellite markers in the abalone Haliotis diversicolor
1Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan Provincial Key Laboratory of Tropical Hydrobiology and Biotechnology, National Class Marine Biology Experiment Teaching Demonstration Center, Ocean College, Hainan University, Haikou, Hainan, China. wy2005@163.com
Abstract:
The abalone, Haliotis diversicolor, is one of the most important mariculture species in southern China. We developed 60 new polymorphic microsatellite markers for H. diversicolor and characterized them in 30 individuals from a cultured population in Sanya, China. All 60 markers were found to be polymorphic. The number of alleles ranged from two to nine per locus, with an average of 4.12/locus. The expected and observed heterozygosities ranged from 0.10 to 0.88 and from 0.07 to 0.87, respectively. Forty-four loci were in Hardy-Weinberg equilibrium. These 44 microsatellite markers should be useful for genome mapping and population genetic studies.
