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[Relationship between Sp3 and the transcriptional regulation of enamelin gene]
Ping Lü1, Hua Tian, Xue-jun Gao
1Department of Cariology and Endodontology, Peking University School, Beijing 100081, China.
Objective:
To investigate the role of Sp3 in the transcriptional regulation of enamelin gene.
Methods:
By bioinformatic analysis, a putative responsive element for Sp3 was identified. Electrophoretic mobility shift assay was used to examine the interaction between Sp3 and enamelin. 5'-flanking regulatory region of enamelin was cloned and ligated into pGL3-basic luciferase vector. Sp3 and the Enam-luc were cotransfected into mouse ameloblast-like cell line, and the activity of luciferase was examined.
Results:
The results showed that Sp3 could not directly bind to the enamelin regulation region and activate enamelin transcription.
Conclusions:
Sp3 might not be involved in transcriptional regulation of enamelin gene via an indirect interaction.
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