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Related Experiment Video

Updated: Aug 5, 2025

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging
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Using mass spectrometry imaging to visualize age-related subcellular disruption.

Kelly A Hogan1,2,3, Julianna D Zeidler4, Heather K Beasley5

  • 1Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA, United States.

Frontiers in Molecular Biosciences
|March 27, 2023
PubMed
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This summary is machine-generated.

Cellular stress disrupts metabolic homeostasis, contributing to aging and age-related diseases. Mass spectrometry imaging offers new ways to study these metabolic changes at the single-cell level.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Gerontology

Background:

  • Metabolic homeostasis is crucial for cellular health.
  • Cellular stress disrupts this balance, contributing to age-related diseases.
  • Organelle dysfunction, including mitochondrial and endoplasmic reticulum (ER) stress, plays a key role in aging.

Purpose of the Study:

  • To explore the role of organelle dysfunction in age-related diseases.
  • To highlight the need for tools to study single-cell metabolic flux in aging.
  • To introduce high-resolution mass spectrometry imaging (MSI) as a solution.

Main Methods:

  • Review of literature on metabolic homeostasis, cellular stress, and aging.
  • Focus on organelle dysfunction (mitochondria, ER) and metabolic flux changes (Ca2+, NAD+).
Keywords:
Golgi complexMERCsagingmass spec imagingmetabolic fluxmitocchondrial dysfunctionspatial omics

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  • Introduction of high-resolution mass spectrometry imaging (MSI) for cellular analysis.

    • Main Results:

    • Organelle dysfunction and altered metabolic flux are implicated in aging.
    • Current tools lack single-cell resolution for metabolite pools and flux.
    • MSI enables high-resolution analysis of cellular metabolism without tissue dissociation.

    Conclusions:

    • Cellular stress and organelle dysfunction are key drivers of aging.
    • MSI is a powerful tool for understanding stress-induced cellular dysfunction in aging.
    • Further research using MSI can elucidate mechanisms of age-related diseases.