Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

P2-engineered exosomes encapsulating curcumin alleviate cognitive decline in AD-like mice by improving microglia-related neuropathology.

Materials today. Bio·2026
Same author

Seizure risk prediction models after intracerebral hemorrhage: a systematic review and meta-analysis.

Neurosurgical review·2026
Same author

High-Performance Transparent Solid Polymer Electrolyte Based on Copolymer of Deep Eutectic Electrolyte and Methyl Methacrylate for Electrochemical Devices.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

NanoBind: Mechanism-Driven Deep Learning of Nanobody-Antigen Molecular Recognition.

Research (Washington, D.C.)·2026
Same author

Deep learning of SHERLOC Raman spectra for facilitating Mars Astromaterial identification.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

Effects of triclopyr on the earthworm (<i>Eisenia fetida</i>) under laboratory conditions: assessment of growth inhibition and oxidative stress.

RSC advances·2026

Related Experiment Video

Updated: Jun 2, 2026

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Observations on novel splice junctions from RNA sequencing data.

Likun Wang1, Xiaowo Wang, Xi Wang

  • 1College of Computer Science and Technology, Jilin University, Changchun 130012, China.

Biochemical and Biophysical Research Communications
|May 18, 2011
PubMed
Summary

SeqSaw is a new tool that improves the detection of novel splice junctions from RNA sequencing (RNA-seq) data. This advancement helps uncover more about gene expression and regulation, even with current sequencing limitations.

More Related Videos

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Related Experiment Videos

Last Updated: Jun 2, 2026

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • High-throughput RNA sequencing (RNA-seq) enables novel transcript discovery.
  • Accurate splice junction identification is crucial but challenging for RNA-seq analysis.
  • Existing methods may miss splice junctions lacking canonical GT-AG signals.

Purpose of the Study:

  • To develop a novel computational tool, SeqSaw, for sensitive and specific splice junction detection.
  • To evaluate SeqSaw's performance against existing methods using real-world RNA-seq datasets.
  • To assess the impact of sequencing depth on novel transcript detection and identify tissue-specific splicing events.

Main Methods:

  • Development of the SeqSaw algorithm for splice junction identification.
  • Application of SeqSaw to ENCODE RNA-seq datasets.
  • Comparative analysis of SeqSaw with two established splice junction detection tools.
  • Analysis of sequencing depth saturation and tissue specificity of splicing events.

Main Results:

  • SeqSaw demonstrated superior performance in identifying novel splice junctions compared to existing methods.
  • Analysis indicated that current sequencing depths are insufficient to reach saturation for novel transcript discovery.
  • A significant number of un-annotated splicing events were found to be tissue-specific.

Conclusions:

  • SeqSaw offers an improved approach for detecting splice junctions, including non-canonical ones.
  • Further increases in sequencing depth are necessary for comprehensive novel transcript identification.
  • Tissue-specific splicing events represent a substantial layer of gene regulation yet to be fully explored.