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The effect of novobiocin on yeast topoisomerase type II
M J Pocklington1, J R Jenkins, E Orr
1Department of Genetics, University of Leicester, England.
Molecular & General Genetics : MGG
|January 1, 1990
Abstract:
Low concentrations of novobiocin are toxic to permeable yeast cells, but do not inhibit type II topoisomerase activity. Furthermore, the enzyme does not bind specifically to novobiocin-Sepharose. These observations are in agreement with genetical analyses. Mutations at a single locus that confer novobiocin resistance and temperature sensitivity exhibit a similar phenotype to cells treated with novobiocin, but are not topoisomerase II mutants.