Related Experiment Videos
Isolation and characterization of protein kinase C from human placenta
C Tertrin-Clary1, M C Chenut, P de la Llosa
1Laboratoire des Hormones Polypeptidiques, CNRS, Gifsur-Yvette Cedex, France.
Placenta
|January 1, 1990
Abstract:
Protein kinase C (Ca2+ and phospholipid-dependent protein kinase) was detected in human placenta and was partially purified using DEAE cellulose chromatography and Ultrogel filtration. Diolein alone did not act on this enzyme but exerted a strong stimulatory action when associated with phosphatidylserine and Ca2+. Similar results were obtained with the phorbol myristate acetate. The kinetic constants for ATP, histone HI or Mg2+ and the apparent Ka for Ca2+ and phosphatidylserine were determined.