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Cell surface glycosyltransferases--do they exist?

W Deppert, G Walter

    Journal of Supramolecular Structure
    |January 1, 1978
    PubMed
    Summary
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    Cell surface glycosyltransferases, crucial for cell recognition, are unlikely to be present on mammalian cells. Assays using intact cells revealed no significant evidence for galactosyltransferases, fucosyltransferases, or sialyltransferases.

    Area of Science:

    • Cell Biology
    • Biochemistry
    • Glycobiology

    Background:

    • Glycosyltransferases are implicated in cell adhesion and recognition.
    • Previous assays for cell surface glycosyltransferases faced challenges including nucleotide sugar hydrolysis and loss of cell integrity.

    Purpose of the Study:

    • To critically re-evaluate the presence and activity of cell surface glycosyltransferases on mammalian cells.
    • To address limitations of previous assay methodologies.

    Main Methods:

    • Assaying intact, viable cells in monolayer cultures.
    • Implementing conditions to prevent intracellular utilization of hydrolyzed nucleotide sugars.

    Main Results:

    • Data strongly suggest the absence of galactosyltransferases on the surface of various mammalian cells (normal, transformed, established, non-established).

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  • No conclusive evidence was found for cell surface fucosyl- and sialyltransferases, though low levels cannot be entirely ruled out.

    • Conclusions:

    • The presence of significant levels of cell surface galactosyltransferases on mammalian cells is unlikely.
    • Further investigation is needed to definitively confirm or refute the presence of low levels of cell surface fucosyl- and sialyltransferases.