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Inhibition of viral mos gene-expression in transformed-cells restricts cell-cycle progression through g1-phase and

R Hamelin1, P Planchon, B Singh

  • 1UNIV TEXAS,MD ANDERSON CANC CTR,DEPT MOLEC BIOL,1515 HOLCOMBE BLVD,HOUSTON,TX 77030. UNIV TEXAS,MD ANDERSON CANC CTR,DEPT MOLEC PATHOL,HOUSTON,TX 77030. INST ONCOL CELLULAIRE & MOLEC HUMAINE,F-93000 BOBIGNY,FRANCE.

International Journal of Oncology
|May 18, 2011
PubMed

Insights

Blocking v-Mos expression in transformed somatic cells halts cell cycle progression, primarily arresting cells in G1 phase and delaying G2 phase. This highlights v-Mos

Area of Science:

  • Cell Biology
  • Molecular Oncology
  • Virology

Background:

  • The c-Mos protein is crucial for M-phase promoting factor (MPF) activation during oocyte maturation and MPF stabilization in unfertilized eggs.
  • The role of viral Mos (v-Mos) in cell cycle progression of transformed somatic cells remains to be fully elucidated.

Purpose of the Study:

  • To investigate the role of v-Mos in cell cycle progression within transformed somatic cells.
  • To determine the specific cell cycle phase(s) affected by the inhibition of v-Mos expression.

Main Methods:

  • Utilized a temperature-sensitive (ts) mutant of v-Mos (MuSV ts110) in NRK-6m2 cells.
  • Generated and employed transformation revertant cell lines (6m3 and 6m4) as controls.
  • Performed flow cytometry analysis with double labeling to quantify cell cycle phases (G0/G1, S, G2/M).

Main Results:

  • NRK-6m2 cells expressing the ts mutant v-Mos failed to express viral mRNA for P85gag-mos at the restrictive temperature (39°C).
  • Blocking v-Mos expression in NRK-6m2 cells at 39°C resulted in a significant growth rate reduction compared to control cells.
  • Flow cytometry revealed that NRK-6m2 cells shifted to 39°C were predominantly arrested in the G1-phase, with a notable delay in G2-phase progression.

Conclusions:

  • v-Mos plays a significant role in regulating cell cycle progression in transformed somatic cells.
  • Inhibition of v-Mos expression leads to G1-phase arrest and delays G2-phase progression, impacting overall cell cycle advancement.

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