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Updated: Jun 1, 2026

Colorectal Cancer Cell Surface Protein Profiling Using an Antibody Microarray and Fluorescence Multiplexing
Published on: September 25, 2011
Epidermal growth-factor receptor and erbb2 protein in colorectal tissue - comparison between cancer and normal mucosa
R Dittadi1, M Gion, A Brazzale
1REG GEN HOSP,CTR STUDY BIOL MARKERS MALIGNANCY,DIV SURG,I-30122 VENICE,ITALY. REG GEN HOSP,DIV SURG,NOALE,ITALY. REG GEN HOSP,DIV SURG,MIRANO,ITALY.
Abstract:
Epidermal growth factor receptor (EGFr) and p185neu protein were measured in 55 samples of carcinoma and 55 of normal colorectal mucosa from the same patient, using a ligand binding assay and an ELISA method respectively. The binding characteristics of EGFr were similar in cancer and normal tissue. The concentrations of both EGFr and p185 showed gaussian distribution and were not significantly different between normal and cancer tissue, although a trend toward higher levels of EGFr in normal mucosa was found. Moreover, no significative variations were found in the ratios between cancer and normal tissue after desaturation of the EGFr. No correlations were found between EGFr and p185 and the main clinopathological parameters.
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