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Isolation and characterization of microsatellite markers from the white-ruffed manakin Corapipo altera (Aves,
Jacob R Barnett1, Laura M Stenzler, Viviana Ruiz-Gutierrez
1Fuller Evolutionary Biology Program, Laboratory of Ornithology, Cornell University, Ithaca, NY 14850, USA, Evolutionary Genetics Core Facility, Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, NY 14853, USA.
Abstract:
We describe 15 polymorphic microsatellite loci from the white-ruffed manakin Corapipo altera, a common understory bird of Neotropical lowland and montane evergreen forests from eastern Honduras to northwestern Colombia. These markers were developed in order to assess population structure and genetic diversity in a fragmented landscape, and to study gene flow between forest fragments. Primers were tested on a population of 159 individuals from the Coto Brus region of southwestern Costa Rica. We found between four and 23 alleles per locus, and observed heterozygosities ranging from 0.23 to 0.93.
