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PERMANENT GENETIC RESOURCES: Polymorphic microsatellite loci for Diachasma alloeum (Hymenoptera: Braconidae)
Andrew A Forbes1, Thomas H Q Powell, Neil F Lobo
1Department of Biological Sciences, 107 Galvin Life Science Building, University of Notre Dame, Notre Dame, IN 46556, USA, Biology Department, Duke University, Research Drive, LSRC B333, Box 90338, Durham, NC 27708, USA.
Abstract:
Here, we report the isolation of 21 novel primers for amplification of microsatellite loci in Diachasma alloeum (Hymenoptera: Braconidae). Diachasma alloeum is a larval parasitoid of the apple maggot fly (Rhagoletis pomonella), which is an economically significant agricultural pest species and a textbook example of sympatric speciation via host-plant shifting. These microsatellite markers will prove useful both for assessing genetic relationships between different host-associated populations of D. alloeum, as well as for future R. pomonella biological control programmes. We also report the cross-species amplification of several loci for Diachasmimorpha mellea and Diachasma ferrugineum, parasitoids of R. pomonella and R. cingulata, respectively.
