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PERMANENT GENETIC RESOURCES: Isolation and characterization of polymorphic microsatellite loci in common evening
E L Larson1, S M Bogdanowicz, A A Agrawal
1Department of Ecology and Evolutionary Biology, Corson Hall, Cornell University, Ithaca, NY 14853, USA, Department of Biology, Duke University, Durham, NC 27708, USA.
Abstract:
We developed nine polymorphic microsatellite loci for evening primrose (Oenothera biennis). These loci have two to 18 alleles per locus and observed heterozygosities ranging from 0 to 0.879 in a sample of 34 individuals. In a pattern consistent with the functionally asexual reproductive system of this species, 17/36 pairs of loci revealed significant linkage disequilibrium and three loci showed significant deviations from Hardy-Weinberg equilibrium. The loci will be informative in identifying genotypes in multigenerational field studies to assess changes in genotype frequencies.
