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Isolation and characterization of 12 dinucleotide microsatellite loci in the anadromous fish Osmerus eperlanus (L.)
A N Taylor1, M I Taylor, I D McCarthy
1School of Ocean Sciences, College of Natural Sciences, University of Wales, Bangor, Menai Bridge, Gwynedd LL59 5AB, UK, School of Biological Sciences, College of Natural Sciences, University of Wales, Bangor, Gwynedd LL57 2UW, UK.
Abstract:
A subtractive hybridization method was used to isolate 12 dinucleotide microsatellite loci for the anadromous European smelt, Osmerus eperlanus (L.). Three to 17 (mean 8.08) alleles per locus were identified in the two populations screened, and observed heterozygosity ranged from 0.067 to 0.933. Loci in both populations showed significant deviations from Hardy-Weinberg expected frequencies. These 12 loci provide a good basis for investigation of O. eperlanus population structure.
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