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Published on: June 2, 2021
Highly polymorphic microsatellite loci in the rice- and maize-infecting fungal pathogen Rhizoctonia solani
M Zala1, B A McDonald, J Bernardes DE Assis
1Plant Pathology, Institute of Integrative Biology, ETH Zurich - Swiss Federal Institute of Technology, Universitaetstrasse 2, LFW B28, 8092 Zurich, Switzerland, UNESP - Universidade Estadual Paulista, Campus de Ilha Solteira, 15385-000 Ilha Solteira, SP, Brazil.
Abstract:
Ten polymorphic microsatellite loci were isolated and characterized from the rice- and maize-infecting Basidiomycete fungus Rhizoctonia solani anastomosis group AG-1 IA. All loci were polymorphic in two populations from Louisiana in USA and Venezuela. The total number of alleles per locus ranged from four to eight. All 10 loci were also useful for genotyping soybean-infecting R. solani AG-1 isolates from Brazil and USA. One locus, TC06, amplified across two other AG groups representing different species, showing species-specific repeat length polymorphism. This marker suite will be used to determine the global population structure of this important pathogenic fungus.
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