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Updated: Jun 1, 2026
Laboratory Protocol for Genetic Gut Content Analyses of Aquatic Macroinvertebrates Using Group-specific rDNA Primers
Published on: October 5, 2017
Microsatellite markers for the endangered Roanoke logperch, Percina rex (Percidae) and their potential utility for
Daniel J Dutton1, James H Roberts, Paul L Angermeier
1*Department of Fisheries and Wildlife Sciences, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA, †Virginia Cooperative Fish and Wildlife Research Unit, US Geological Survey, ‡Blacksburg, VA 24061, USA.
Abstract:
The Roanoke logperch (Percina rex Jordan and Evermann), an endangered fish, occurs in only six watersheds in the Roanoke and Chowan river drainages of Virginia, USA. The species' population genetic structure is poorly known. We developed 16 microsatellite markers that were reliably scorable and polymorphic P. rex. Markers were also screened in seven other darter species of the genus Percina. Most markers exhibited successful amplification and polymorphism in several species. These markers may therefore prove useful for population genetic studies in other darters, a diverse but highly imperiled group.
