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Published on: October 5, 2017
Cross-amplification and characterization of 13 tetranucleotide microsatellites in multiple species of Neotropical
Manoel L DA Fontoura-Rodrigues1, Carlos André V Lima-Rosa, Ligia Tchaicka
1Faculdade de Biociências, PUCRS, Av. Ipiranga 6681, Prédio 12, Porto Alegre, RS 90619-900, Brazil.
Abstract:
We tested 47 tetranucleotide microsatellite loci developed for the domestic dog in four species of Neotropical canids, aiming to produce a standardized set that could be successfully used even in noninvasive samples across this group. We identified 13 suitable loci, nine of which constitute a standardized set for all species. Considering only the ideal panel of nine loci, the mean expected heterozygosity (averaged across species) per locus ranged from 0.58 to 0.92 (overall mean 0.76), and the maximum probability of identity value was 1.3 × 10(-9) . This set of loci has a great potential for application in evolutionary, ecological and conservation studies.