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Microsatellite markers for the drought-resistant earthworm Hormogaster elisae
M Novo1, T P Velavan, A Almodóvar
1Department of Zoology, Complutense University of Madrid, Madrid, Spain, Department of Animal Evolutionary Ecology, University of Tüebingen, Tüebingen, Germany.
Molecular Ecology Resources
|May 19, 2011
Summary
We developed new genetic markers for the earthworm Hormogaster elisae. These microsatellite loci show high variability and will aid in population genetics and paternity analysis.
Area of Science:
- Genomics
- Zoology
- Ecology
Background:
- The earthworm Hormogaster elisae is an important soil organism.
- Understanding its population genetics is crucial for ecological studies.
Purpose of the Study:
- To develop and characterize novel microsatellite loci for Hormogaster elisae.
- To provide genetic tools for future population and paternity analyses.
Main Methods:
- Developed 10 microsatellite loci from a simple sequence repeat-enriched genomic DNA library.
- Characterized these loci in 26 individuals of Hormogaster elisae.
Main Results:
- All 10 loci were polymorphic.
- Observed 8 to 25 alleles per locus.
- High levels of heterozygosity were detected.
Conclusions:
- The developed microsatellite loci are suitable for population genetic studies.
- These markers will be valuable for paternity analysis in Hormogaster elisae.
