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Updated: Jun 1, 2026
Microsatellite DNA Genotyping and Flow Cytometry Ploidy Analyses of Formalin-fixed Paraffin-embedded Hydatidiform Molar Tissues
Published on: October 20, 2019
Microsatellite loci isolated from the tropical tree Hymenaea courbaril L. (Fabaceae)
Anna Buonamici1, Stephen Cavers, G G Vendramin
1Istituto di Genetica Vegetale, Consiglio Nazionale delle Ricerche, Via Madonna del Piano 10, 50019 Sesto Fiorentino (Firenze), Italy, Centre for Ecology & Hydrology, Bush Estate, Penicuik, Midlothian, EH26 0QB, UK.
Abstract:
We developed 11 microsatellite markers for Hymenaea courbaril for the purpose of studying spatial genetic structure and gene flow. The microsatellite loci were screened in 44 trees from two populations. All loci were polymorphic, exhibiting between two and 16 alleles, and levels of expected heterozygosity from 0.174 to 0.909. Departures from Hardy-Weinberg equilibrium were detected for all loci in one population. The estimated null allele frequency is low or moderate. No locus combinations exhibited linkage disequilibrium.
