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Isolation and characterization of 12 polymorphic microsatellite markers in the tropical house gecko (Hemidactylus
Kristen Harfmann Short1, Kenneth Petren
1Department of Biological Sciences, University of Cincinnati, Cincinnati, OH 45221-0006, USA.
Abstract:
We present 12 variable microsatellite loci isolated from the invasive tropical house gecko, Hemidactylus mabouia. Polymerase chain reaction (PCR) primers were tested on 39 individuals from two locations in Miami, Florida. Heterozygote deficiency was detected for four loci, and we attribute this to possible null alleles or population substructure. Some loci successfully amplified PCR products in several congeners, indicating their potential for use in other geckos.
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