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Development of microsatellite markers in Annona crassiflora Mart., a Brazilian Cerrado fruit tree species
M F Pereira1, L F Bandeira, A J V Blanco
1Graduate Program in Agronomy (Genetics and Plant Breeding), Graduate Program in Molecular and Cell Biology, Universidade Federal de Goiás, Campus Samambaia, Goiânia, GO, 74001-970, PO Box 131, Brazil, Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte, Brazília, DF, 70770 900, PO Box 02372, Brazil.
Abstract:
Annona crassiflora Mart. (Annonaceae) is a native fruit species of the region of Brazilian Cerrado with a high agronomic potential, although without any traces of domestication. A set of 10 microsatellite primer pairs was developed from an enriched genome library (TC13). An average of 19.3 alleles per locus was detected. Observed heterozygosity estimates were consistently lower than those obtained for gene diversity, evidencing a departure from Hardy-Weinberg expected proportions. The reported set of markers showed to be highly informative and constitutes a powerful tool for the development of genetic characterization studies in A. crassiflora.
