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An Improved Method of RNA Isolation from Loblolly Pine (P. taeda L.) and Other Conifer Species
Published on: February 22, 2010
Isolation and characterization of microsatellite markers for Carolina hemlock (Tsuga caroliniana)
S A Josserand1, K M Potter, C S Echt
1US Department of Agriculture Forest Service, Southern Research Station, Southern Institute of Forest Genetics, 23332 Mississippi 67, Saucier, MS 39574, USA, North Carolina State University, 3041 Cornwallis Road, Research Triangle Park, NC 27709, USA.
Abstract:
We describe the isolation and characterization of 31 polymorphic di- and trinucleotide microsatellite marker loci for Carolina hemlock (Tsuga caroliniana Englem.). In addition, primer pairs for 16 loci amplified scoreable alleles in six other Tsuga species. In eastern North America, both Carolina hemlock and eastern hemlock (Tsuga canadensis [L.] Carr.) populations are declining due to infestation by hemlock woolly adelgid, Adelges tsugae. The markers described here should enhance population genetic studies of hemlocks, providing valuable information for conserving and restoring these important forest tree species.
