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Published on: October 5, 2017
Isolation and characterization of microsatellite marker loci in the greylag goose (Anser anser)
B M Weiß1, K Poggemann, K Olek
1Konrad Lorenz Forschungsstelle, A-4645 Grünau im Almtal, Austria, Certagen GmbH, D-53359 Rheinbach, Germany, Biopsytec Analytik GmbH, D-53359 Rheinbach, Germany, Institute of Biology, University of Neuchâtel, CH-2009 Neuchâtel, Switzerland.
Abstract:
Ten novel polymorphic microsatellite loci were isolated and characterized from the greylag goose, Anser anser, a long-term monogamous and biparental bird. Additionally, five new primers pairs were designed based on previously published microsatellite locus sequences from closely related species. Multiplex polymerase chain reactions conditions were optimized for all 15 primer pairs. The number of alleles ranged from two to 12 per locus with an observed heterozygosity ranging from 0.07 to 0.85. This marker set will be used to determine rates and origins of extra-pair and parasitic young in a population of individually banded greylag geese with known life histories.
