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Characterization of new microsatellite markers derived from sequence databases for the emu (Dromaius novaehollandiae)
José M Yáñez1, Ruth González, Jenniffer Angulo
1Animal Breeding and Genomics Laboratory, Universidad de Chile, Santa Rosa 11735, Santiago, Chile, Laboratory of Molecular Ecology and Evolutionary Studies, Universidad de Santiago de Chile, Avenida Libertador Bernardo O'Higgins 3363, Santiago, Chile, Nutrition, Diabetes, and Metabolism Department, Pontificia Universidad Católica de Chile, Marcoleta 367, Santiago, Chile.
Abstract:
The emu (Dromaius novaehollandiae), a member of ratite family, is native to Australia and has been introduced to other countries worldwide. In this work, 10 polymorphic microsatellite loci were isolated and characterized for emu from public sequences. Polymorphism was surveyed in 22 individuals from two different populations kept in captivity. Between two and 11 alleles were found per locus, and the observed heterozygosity ranged from 0.05 to 0.85, in accordance with expectations. These markers will be useful as tools for detecting levels of genetic variation, reconstructing pedigrees (for quantitative genetic analysis) and identifying markers associated to fitness traits in emu populations.
