Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

Protein Folding Quality Check in the RER

Protein Folding Quality Check in the RER

ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...

Calmodulin-dependent Signaling

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

The Calvin Benson Cycle

The Calvin Benson Cycle

Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...

Cell Adhesion in Plants

Cell Adhesion in Plants

Plants have rigid cell walls that are made up of cell wall polysaccharides that mediate cell-cell adhesion. The primary cell walls of plants consist of two independent and interacting polysaccharide networks: a pectin matrix that embeds the second network comprising cellulose and hemicelluloses.
Pectins are complex heteropolymers mainly composed of negatively-charged α-D-glucopyranosyl uronic acid and some neutral glycosyl residues such as α-L-rhamnopyranose, α-L-arabinofuranose, and...

Regulation of the Unfolded Protein Response

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reimagining plant science training in the era of generative artificial intelligence: a global perspective.

The Plant cell·2026

Same author

Constructing gene co-functional and co-regulatory networks from public transcriptomes using condition-specific ensemble co-expression.

Nature communications·2026

Same author

Investigating the crystalline structure and structural heterogeneity of starch granules using polarization-based quantitative phase microscopy.

Food chemistry·2026

Same author

Differential cellulose distribution drives polarized growth of cotton fibers.

Nature communications·2026

Same author

GraFT: A robust network-based spatiotemporal analysis of filamentous structures.

Science advances·2026

Same author

Turgor reduction triggers FERONIA nanodomain assembly for osmosensing in plants.

Current biology : CB·2026

Search research articles

Related Experiment Video

Updated: Jun 1, 2026

Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling

Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling

Published on: August 1, 2020

Diverging functions among calreticulin isoforms in higher plants.

Lisa Thelin¹, Marek Mutwil, Marianne Sommarin

¹Center for Molecular Protein Science, Biochemistry and Biophysical Chemistry, Lund University, Lund, Sweden.

Plant Signaling & Behavior

|May 19, 2011

Summary

Plant calreticulins show functional divergence, with one isoform specializing in receptor folding. This discovery suggests potential functional specialization for calreticulin isoforms in animal cells too.

More Related Videos

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on ¹⁵N Metabolic Labeling and Quantitative Mass Spectrometry

Published on: March 13, 2014

Related Experiment Videos

Last Updated: Jun 1, 2026

Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling

Forward Genetic Screen Using Transgenic Calcium Reporter Aequorin to Identify Novel Targets in Calcium Signaling

Published on: August 1, 2020

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on 15N Metabolic Labeling and Quantitative Mass Spectrometry

A New Approach for the Comparative Analysis of Multiprotein Complexes Based on ¹⁵N Metabolic Labeling and Quantitative Mass Spectrometry

Published on: March 13, 2014

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Calreticulin is an ER chaperone involved in cellular processes like calcium homeostasis, apoptosis, and cell adhesion.
Research on animal calreticulins has primarily focused on a single isoform, overlooking potential functional diversity.

Purpose of the Study:

To explore the functional divergence of calreticulin isoforms.
To investigate the specialized roles of plant calreticulin isoforms.

Main Methods:

Comparative analysis of calreticulin isoforms across species.
Functional characterization of plant calreticulin isoforms.

Main Results:

Two plant calreticulin isoforms function within the general ER chaperone system.
A third plant calreticulin isoform is specifically associated with the folding of brassinosteroid and bacterial peptide receptors.

Conclusions:

Plant calreticulins exhibit functional specialization, indicating diverse roles beyond general chaperone activity.
The discovery of specialized plant calreticulins opens new avenues for investigating isoform-specific functions in animal cells.