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Nasal potential difference measurements to assess CFTR ion channel activity

Steven M Rowe1, John Paul Clancy, Michael Wilschanski

  • 1Department of Medicine, University of Alabama, Birmingham, AL 35294-0006, USA. smrowe@uab.edu

Methods in Molecular Biology (Clifton, N.J.)
|May 20, 2011
PubMed

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Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...