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Published on: September 21, 2017
Determination of daumone in mouse plasma by HPLC/MS-MS
Keumhan Noh1, Jong Hee Park, Minkyu Kim
1College of Pharmacy, Yeungnam University, Kyoungsan, Kyoungbuk 712-749, South Korea.
Biomedical Chromatography : BMC
|May 20, 2011
Summary
A new method accurately quantifies daumone, a key pheromone regulating aging and chemosensation. This technique was used to measure daumone levels in mice after repeated oral administration.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Neuroscience
Background:
- Daumone, a Caenorhabditis elegans pheromone, plays a crucial role in regulating chemosensory processes.
- Understanding daumone's biological roles requires reliable methods for its quantification in biological matrices.
Purpose of the Study:
- To develop and validate a sensitive and accurate method for quantifying daumone in mouse plasma.
- To apply the developed method to assess plasma daumone concentrations after repeated oral administration in mice.
Main Methods:
- High-performance liquid chromatography coupled with tandem mass spectrometry (HPLC/MS-MS) was employed for daumone determination.
- Simple protein precipitation with acetonitrile and methaqualone (internal standard) was used for sample preparation.
- Reversed-phase chromatography and electrospray ionization were utilized for analyte separation and detection.
Main Results:
- The developed HPLC/MS-MS assay demonstrated high accuracy and precision, meeting FDA bioanalytical validation guidelines.
- The method was successfully applied to measure plasma daumone concentrations in mice.
Conclusions:
- A validated HPLC/MS-MS method provides a reliable tool for daumone quantification in mouse plasma.
- This method enables further investigation into daumone's pharmacokinetic and pharmacodynamic properties in vivo.
