Related Experiment Video
Updated: Jun 1, 2026
03:57
Generation of Hypoparathyroid Rats via Carbon-Nanoparticle-Assisted Parathyroidectomy
Published on: July 14, 2023
Parathyroid hormone control survey to determine inter-method and inter-lab variations in Japan
Takatoshi Kakuta1, Hitoshi Kato, Hirotaka Komaba
1Division of Nephrology, Endocrinology and Metabolism, Tokai University School of Medicine, Isehara, Kanagawa, Japan. kakuta@is.icc.u-tokai.ac.jp
Summary
Different parathyroid hormone (PTH) assays show significant variations in results for uremic patients. These inter-method differences impact the accurate assessment of PTH levels, crucial for managing kidney disease.
Area of Science:
- Clinical Chemistry
- Nephrology
- Biomarker Assays
Background:
- Kidney Disease: Improving Global Outcomes (KDIGO) guidelines recommend specific parathyroid hormone (PTH) levels for uremic patients.
- Accurate PTH measurement is essential for managing secondary hyperparathyroidism in chronic kidney disease.
- Limitations in available PTH assays hinder precise therapeutic target attainment.
Purpose of the Study:
- To evaluate the suitability of various commercial immunoassays for measuring parathyroid hormone (PTH) in uremic patients.
- To assess inter-method variability among different PTH assays used in Japan.
- To identify potential discrepancies in PTH quantification relevant to clinical guidelines.
Main Methods:
- Comparison of five commercial parathyroid hormone (PTH) immunoassays.
- Utilized three serum pools and seven artificially spiked samples for validation.
- Employed the Total Intact PTH assay as the reference method for analysis.
Main Results:
- High correlation observed across different parathyroid hormone (PTH) assays, but significant quantitative differences were noted.
- A notable variation, approximately 60%, was found in the ratio between third-generation and second-generation PTH assays.
- The Immulite assay demonstrated an overestimation of 1-84 PTH levels.
Conclusions:
- Significant inter-method variations exist among parathyroid hormone (PTH) assays currently used in Japan.
- These assay discrepancies pose challenges in achieving KDIGO-recommended PTH targets for uremic patients.
- Further standardization or careful assay selection is necessary for reliable PTH monitoring in nephrology practice.
Related Concept Videos
00:59The Parathyroid Glands
The two pairs of parathyroid glands embedded within the posterior surface of the thyroid gland are restricted by a dense capsule around them. These glands comprise two distinct cell populations—parathyroid oxyphil and parathyroid principal cells- pivotal in calcium homeostasis.
Oxyphil cells, whose functions remain elusive, emerge during late puberty, adding a layer of complexity to the parathyroid gland's intricacies. In contrast, principal parathyroid cells undertake a vital role by producing...
Oxyphil cells, whose functions remain elusive, emerge during late puberty, adding a layer of complexity to the parathyroid gland's intricacies. In contrast, principal parathyroid cells undertake a vital role by producing...
01:17Hormones and Bone Tissue
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
00:51Synthesis and Functions of Calcitonin
Calcitonin, a vital polypeptide hormone, regulates calcium levels within body fluids. It is released by the parafollicular cells, also known as C cells, situated in the follicular epithelium of the thyroid gland. Calcitonin responds to fluctuations in blood calcium levels and the influence of gastrointestinal hormones like gastrin and cholecystokinin.
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...
01:20Synthesis and Regulation of Thyroid Hormones
Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...
01:33Hormonal Regulation
The renin-aldosterone system is an endocrine system which guides the renal absorption of water and electrolytes, thus managing blood pressure and osmoregulation. Activation of the system begins in the kidneys with a small cluster of cells adjacent to the afferent and efferent blood vessels of the renal corpuscle. As the nephrons are filtering blood, juxtaglomerular cells monitor blood pressure. If they detect a decrease in pressure, they release the hormone renin into the bloodstream.
01:40Hormonal Regulation
Hormones regulate a significant portion of digestion through activation of the neuroendocrine system. The neuroendocrine system of digestion contains many different hormones all with multiple functions that are both, directly and indirectly, involved in digestion.