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Updated: Jun 1, 2026
Cell Type-specific Gene Expression Profiling in the Mouse Liver
Published on: September 17, 2019
The expression of TIPE1 in murine tissues and human cell lines
Jian Cui1, Guizhong Zhang, Chunyan Hao
1Shandong University School of Medicine, Ji'nan, PR China.
Abstract:
Members of the tumor necrosis factor-alpha-induced protein-8 (TNFAIP8 or TIPE) family play important roles in immune homeostasis and cancer. TIPE1 (TNFAIP8-like 1) is a new member of the TIPE family that may regulate cell death. However, due to the lack of a suitable antibody, the nature of cells and tissues that express TIPE1 protein has not been determined. In this study, we generated a highly specific antibody to TIPE1 and examined TIPE1 expression in various murine tissues and human cell lines by immunohistochemistry, reverse transcription real-time PCR, and Western blot. We found that TIPE1 protein was detected in a wide variety of tissues in C57BL/6 mice, such as neurons in brain, hepatocytes, germ cells of female and male reproductive organs, muscular tissues, and a variety of cells of the epithelial origin, particularly those with secretory functions. TIPE1 protein was not expressed in mature T or B lymphocytes, but detectable in human B lymphoblast cell line HMy2.CIR and murine T cell line EL4. Furthermore, high levels of TIPE1 mRNA were detected in most human carcinoma cell lines, especially in cells transformed with viral genomes. These results indicate that TIPE1 may perform functions in cell secretion and carcinogenesis, but not in immunity.
Insights
Tumor necrosis factor-alpha-induced protein-8-like 1 (TIPE1) is found in many mouse tissues, including neurons and epithelial cells. TIPE1 protein is not in immune cells but may play roles in cell secretion and cancer development.
Area of Science:
- Immunology
- Cell Biology
- Oncology
Background:
- The tumor necrosis factor-alpha-induced protein-8 (TNFAIP8 or TIPE) family is crucial for immune balance and cancer.
- TIPE1 (TNFAIP8-like 1) is a TIPE family member potentially involved in cell death regulation.
- Previous studies lacked specific antibodies, hindering TIPE1 expression analysis.
Purpose of the Study:
- To generate a specific antibody for TIPE1.
- To investigate TIPE1 protein and mRNA expression across various murine tissues and human cell lines.
Main Methods:
- Immunohistochemistry
- Reverse transcription real-time PCR (RT-PCR)
- Western blot analysis
Main Results:
- TIPE1 protein is widely expressed in mouse tissues, including brain neurons, hepatocytes, germ cells, muscle, and secretory epithelial cells.
- TIPE1 is absent in mature T and B lymphocytes but detected in specific cell lines (HMy2.CIR, EL4).
- High TIPE1 mRNA levels are observed in human carcinoma cell lines, particularly those with viral transformation.
Conclusions:
- TIPE1 protein expression is widespread in non-immune tissues.
- TIPE1 may be involved in cellular secretion and carcinogenesis.
- TIPE1 does not appear to play a significant role in immunity.
