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Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...
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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

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Related Experiment Video

Updated: Jun 1, 2026

Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos
09:20

Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

Published on: May 14, 2019

One for all--a highly efficient and versatile method for fluorescent immunostaining in fish embryos.

Daigo Inoue1, Joachim Wittbrodt

  • 1Centre for Organismal Studies, University of Heidelberg, Heidelberg, Baden-Württemberg, Germany. daigo.inoue@cos.uni-heidelberg.de

Plos One
|May 24, 2011
PubMed
Summary

A new standard protocol simplifies antigen retrieval for fluorescent immunostaining in zebrafish and medaka. This method enhances tissue preservation and enables multi-color and co-staining applications.

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Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae
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Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos
09:20

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Published on: May 14, 2019

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence
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Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae
07:29

Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae

Published on: January 29, 2020

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Immunohistochemistry

Background:

  • Immunostaining is crucial for detecting proteins in tissues and whole organisms.
  • Current methods require specific protocols for each antibody, making optimization difficult and time-consuming.

Purpose of the Study:

  • To develop a novel, widely applicable standard protocol for antigen retrieval.
  • To facilitate fluorescent immunostaining in various sample types.

Main Methods:

  • A new, standardized antigen retrieval method was developed.
  • The protocol was tested on cryosections and whole mount preparations.

Main Results:

  • The novel method efficiently retrieves antigens using a single, widely applicable protocol.
  • Successful fluorescent immunostaining was achieved in zebrafish (Danio rerio) and medaka (Oryzias latipes).

Conclusions:

  • The method prevents loss of tissue sections and damage to morphology.
  • It enables parallel multi-color immunostaining, co-immunostaining with fluorescent proteins, and integration with whole mount in situ hybridization.