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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
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Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry

Published on: July 31, 2011

A case study on the comparison of different software tools for automated quantification of peptides

Niklaas Colaert1, Joël Vandekerckhove, Lennart Martens

  • 1Department of Medical Protein Research, VIB, Ghent University, B-9000, Ghent, Belgium. niklaas.colaert@vib-ugent.be

Methods in Molecular Biology (Clifton, N.J.)
|May 24, 2011
PubMed

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Peptide Identification Using Tandem Mass Spectrometry01:33

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...