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Updated: Jun 1, 2026

A Simple Method for Isolation of Soybean Protoplasts and Application to Transient Gene Expression Analyses
Published on: January 25, 2018
Molecular characterization of a new Tospovirus infecting soybean
J Zhou1, S K Kantartzi, R-H Wen
1Division of Agriculture, Department of Plant Pathology, University of Arkansas, 495 N. Campus Dr., 217 Plant Sciences Building, Fayetteville, 72701, USA.
Abstract:
A new, widespread disease was recently observed in soybean in the United States. The disease, named Soybean vein necrosis, is manifested by intraveinal chlorosis and necrosis, and has been found in almost all of the 50 fields visited over a period of 3 years in the midwest and midsouth part of the United States. A virus was isolated from symptomatic material, and detection protocols were developed. More than 150 symptomatic specimens collected from seven US States were tested, and all were found positive for the virus unlike 75 asymptomatic samples, revealing the absolute association between virus and disease. Protein pairwise comparisons coupled with phylogenetic analyses indicate that the virus is a new member of the genus Tospovirus.
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