Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Functional Divergence and Structural Changes of class IV Histone Deacetylases (HDACs) Across the Tree of Life.

Molecular biology and evolution·2026

Same author

Macrophage-Centric Phenotypic Screening Identifies Tetrazolone-Based HDAC6 Inhibitors That Reprogram the Tumor Immune Microenvironment and Improve Immune Checkpoint Blockade.

Journal of medicinal chemistry·2026

Same author

Beyond formic acid: Peptides in carpenter ant venoms aid in disease protection.

Science advances·2026

Same author

Capsaicin-Inspired Hydroxamate Hybrids as Selective HDAC6 Inhibitors with Antiproliferative Activity in Hematological Malignancies.

ACS omega·2026

Same author

Selective targeting of cortactin tandem repeat acetylation by human lysine deacetylases.

The FEBS journal·2026

Same author

25-Hydroxyvitamin D<sub>3</sub> metabolism modulates the effect of variable UV exposure on 25-hydroxyvitamin D<sub>3</sub> plasma concentrations.

Communications medicine·2026

Same journal

Optimizing miRNA Loading into Platelet Exosomes via Electroporation: A Comparative Analysis of Buffer Systems and Voltage Parameters for Enhanced Targeted Drug Delivery.

Molecular biotechnology·2026

Same journal

Regulation of Goat Milk Protein Synthesis: Genetic Architecture, Signalling Pathways, and Omics Insights.

Molecular biotechnology·2026

Same journal

Cloning and Functional Characterization of AhyAP-T65Lig, an ATP-Dependent DNA Ligase from Trabzonvirus AP-T65.

Molecular biotechnology·2026

Same journal

Overexpression of the ATP-Citrate Lyase Gene Enhances Ganoderic Acid Biosynthesis in Ganoderma lingzhi.

Molecular biotechnology·2026

Same journal

CRISPR/Cas9 Mediated Genome Editing for Enhancing Abiotic Stress Tolerance in Rice: An Omics Guided Perspective.

Molecular biotechnology·2026

Same journal

Functionalization of Chitosan with Silver Oxide and Berberis aristata for Bioactive Composites with In Vivo Wound Healing Evaluation.

Molecular biotechnology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 1, 2026

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Deciphering enzyme function using peptide arrays.

Alexandra Thiele¹, Gabriele I Stangl, Mike Schutkowski

¹Institute of Agricultural and Nutritional Sciences, Department of Human Nutrition, Martin-Luther-University Halle-Wittenberg, Von Danckelmann Platz 2, 06120 Halle, Germany.

Molecular Biotechnology

|May 24, 2011

Summary

This summary is machine-generated.

Peptide microarrays efficiently profile enzyme activity and substrate specificity for kinases, proteases, and phosphatases. This technology aids in identifying enzyme targets and optimizing substrates for signal-transduction pathways.

More Related Videos

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

Peptide-based Identification of Functional Motifs and their Binding Partners

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

Related Experiment Videos

Last Updated: Jun 1, 2026

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Published on: November 29, 2014

Peptide-based Identification of Functional Motifs and their Binding Partners

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

Area of Science:

Biochemistry and Molecular Biology
Enzymology
Proteomics

Background:

Enzymes, including kinases, proteases, and phosphatases, are crucial for cellular signaling.
Characterization of human enzymes remains incomplete, hindering a full understanding of signal-transduction pathways.
High-throughput screening (HTS) assays are essential for enzyme profiling but can be time-consuming.

Purpose of the Study:

To review assay principles for measuring enzyme activities (kinases, phosphatases, proteases).
To highlight the application of peptide microarrays for enzyme substrate identification and optimization.
To summarize the use of peptide arrays for profiling enzyme activities in complex biological samples.

Main Methods:

Utilizing peptide microarray-based technologies for high-throughput enzyme profiling.
Developing and applying assay principles for measuring kinase, protease, and phosphatase activities.
Employing high-density peptide microarrays for simultaneous profiling in biological lysates.

Main Results:

Peptide microarrays significantly reduce assay setup time and enable efficient profiling of enzyme substrate specificity.
Successful identification and optimization of enzyme substrates for various enzyme classes, including kinases, lysine methyl-transferases, histone deacetylases, and SUMO-transferases.
Demonstrated utility of peptide arrays for simultaneous profiling of kinase activities in complex samples like cell and organism lysates.

Conclusions:

Peptide microarray technology offers a powerful and efficient approach for characterizing enzyme activities and substrate specificities.
This technology accelerates the discovery of enzyme targets and facilitates the optimization of enzyme substrates.
Peptide arrays are valuable tools for comprehensive enzyme profiling in diverse biological contexts, advancing our understanding of signal transduction.