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Divergent overlapping transcripts at the PET122 locus in Saccharomyces cerevisiae
J D Ohmen1, K A Burke, J E McEwen
1Department of Microbiology, University of California, Los Angeles 90024.
Molecular and Cellular Biology
|June 1, 1990
Summary
This study identifies PET122, ORF2, and ORF3 genes in yeast, revealing overlapping antisense transcripts. These overlapping transcripts suggest a novel mechanism for interdependent post-transcriptional gene regulation in mitochondria.
Area of Science:
- Molecular Biology
- Yeast Genetics
- Mitochondrial Gene Expression
Background:
- PET122 is crucial for translating mitochondrial mRNA of cytochrome c oxidase subunit III in Saccharomyces cerevisiae.
- The PET122 locus contains PET122, ORF2, and ORF3, with complex transcript production.
Purpose of the Study:
- To elucidate the genetic organization and transcriptional regulation at the PET122 locus.
- To investigate the potential for interdependent post-transcriptional regulation.
Main Methods:
- DNA sequencing of the PET122 locus.
- Transcript analysis to identify gene products and sizes.
- Analysis of transcript overlaps and antisense relationships.
Main Results:
- The PET122 locus spans 2,862 bp, containing PET122 (254 codons), ORF2, and ORF3.
- Five transcripts (2.9, 2.3, 2.1, 1.5, 1.4 kb) are produced, encoding PET122, ORF2, and ORF3.
- Significant overlaps exist between PET122 and ORF3 transcripts, forming antisense pairs.
Conclusions:
- The PET122 locus exhibits a complex gene arrangement with overlapping antisense transcripts.
- These overlapping transcripts suggest a novel regulatory mechanism for PET122 and ORF3 expression.