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Centrifugation01:05

Centrifugation

Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
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Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
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Cell clarification and size separation using continuous countercurrent magnetophoresis.

Seyda Bucak1, Sonja Sharpe, Simon Kuhn

  • 1Dept. of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. seyda@yeditepe.edu.tr

Biotechnology Progress
|May 25, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a continuous magnetophoretic countercurrent separation technique using ferrofluids to remove micron-sized particles from suspensions. This method offers an efficient approach for cell clarification in raw fermentation broth.

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Area of Science:

  • Biophysics
  • Separation Science
  • Biotechnology

Background:

  • Nonmagnetic microparticles in ferrofluids experience magnetophoretic forces in nonuniform magnetic fields.
  • This force drives particles towards regions of lower magnetic field strength.
  • This phenomenon can be leveraged for particle separation and concentration.

Purpose of the Study:

  • To develop a continuous magnetophoretic countercurrent separation method.
  • To demonstrate its application in removing and concentrating micron-sized particles from aqueous suspensions.
  • To validate its viability for cell clarification of raw fermentation broth.

Main Methods:

  • A ferrofluid is mixed with the particle suspension (e.g., cells).
  • The mixture flows through an open tube with moving magnet pairs.
  • Magnets move counter to the suspension flow, creating a magnetic field gradient.

Main Results:

  • Magnetophoretic forces propel microparticles ahead of the moving magnet pairs.
  • Particles are collected upstream of the injection point.
  • A concentrated suspension of particles is removed for further processing.

Conclusions:

  • Continuous magnetophoretic countercurrent separation is effective for micron-sized particle removal.
  • This technique provides a viable method for cell clarification in fermentation processes.
  • The approach facilitates efficient concentration of target particles from suspensions.