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Production of Large Numbers of Size-controlled Tumor Spheroids Using Microwell Plates
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The 104-well microplate.

Mark G Herrmann1, Tanya M Sandrock

  • 1Institute for Clinical and Experimental Pathology, ARUP Laboratories, Salt Lake City, UT 84108, USA. mark.herrmann@aruplab.com

Journal of Laboratory Automation
|May 26, 2011
PubMed
Summary
This summary is machine-generated.

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A new 104-well microplate offers a simple solution for labs needing extra space for standards and controls. This 104-well plate integrates seamlessly into existing 96-well workflows without impacting results.

Area of Science:

  • Biotechnology
  • Laboratory Science
  • Assay Development

Background:

  • The standard 96-well microplate format can be restrictive, limiting space for additional standards or controls.
  • Splitting plates or reducing sample numbers leads to increased costs and inefficiencies.

Purpose of the Study:

  • To develop and validate a novel 104-well microplate as a cost-effective alternative to the traditional 96-well format.
  • To provide additional capacity for standards and controls without altering experimental protocols or requiring new equipment.

Main Methods:

  • A new 8 × 13-array microplate (104-well) was designed adhering to ANSI/SBS standards, with a shifted 13th column.
  • Absorbance readings were compared between 96- and 104-well formats to assess optical density differences.

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  • The 104-well plate was integrated into a standard liquid handler for an enzyme-linked immunosorbent assay (ELISA).

    • Main Results:

    • Negligible differences in mean absorbance readings (-0.0003 optical density) were observed between the 96- and 104-well formats.
    • Assay results from the 104-well plate showed no significant difference compared to the 96-well format (r=0.997).
    • The 104-well plate demonstrated successful integration into a standard liquid handling system for ELISA.

    Conclusions:

    • The 104-well microplate provides a viable solution for increasing sample or control capacity within existing laboratory workflows.
    • This novel plate format can be incorporated into 96-well environments without significant changes to current systems or experimental outcomes.
    • The 104-well plate offers a practical and cost-effective enhancement for various laboratory applications, including ELISA.