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Related Experiment Video

Updated: Jun 1, 2026

Automated Robotic Liquid Handling Assembly of Modular DNA Devices
11:22

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

Published on: December 1, 2017

Adding precise nanoliter volume capabilities to liquid-handling automation for compound screening experimentation.

Tal V Murthy1, Doug Kroncke, Paul D Bonin

  • 1Applications Laboratories, Thermo Fisher Scientific, Hudson, NH 03051, USA. tal.murthy@thermofisher.com

Journal of Laboratory Automation
|May 26, 2011
PubMed
Summary

PocketTips enable economical nanoliter-scale compound screening by precisely holding volumes, reducing reliance on expensive, bulky liquid-handling automation. This technology offers consistent, instrument-independent dispensing for high-throughput screening.

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Last Updated: Jun 1, 2026

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Area of Science:

  • Biotechnology
  • Chemical Biology
  • Assay Development

Background:

  • Nanoliter-scale compound screening is cost-effective for high-throughput experiments.
  • Current nanoliter fluid handling automation is often expensive, bulky, and requires calibration.
  • Instrument-to-instrument variability in low-volume dispensing is a persistent challenge.

Purpose of the Study:

  • To introduce and evaluate PocketTips as a novel solution for nanoliter fluid handling.
  • To demonstrate the performance of PocketTips across different automation platforms.
  • To validate the accuracy and reliability of PocketTips in compound delivery for cell-based assays.

Main Methods:

  • Utilized PocketTips, featuring a defined internal pocket for precise nanoliter volume retention.
  • Tested PocketTip performance on various liquid-handling automation platforms.
  • Employed a cell-based beta-lactamase reporter assay to compare PocketTip delivery with standard methods.

Main Results:

  • PocketTips demonstrated consistent nanoliter volume dispensing, independent of the liquid-handling device's inherent capabilities.
  • Performance was validated across multiple automation platforms.
  • Compound delivery using PocketTips compared favorably to standard compound addition techniques in a cell-based assay.

Conclusions:

  • PocketTips offer an economical and reliable method for nanoliter fluid handling in compound screening.
  • This technology mitigates issues associated with expensive automation and instrument variability.
  • PocketTips provide a robust solution for accurate compound delivery in high-throughput screening applications.