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Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Cadherins in Tissue Organization01:19

Cadherins in Tissue Organization

The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
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Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
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Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
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Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
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Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

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Related Experiment Video

Updated: Jun 1, 2026

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

Epithelial cell organization suppresses Myc function by attenuating Myc expression.

David R Simpson1, Min Yu, Siyuan Zheng

  • 1Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA.

Cancer Research
|May 26, 2011
PubMed
Summary
This summary is machine-generated.

Cellular organization in mammary tissues suppresses oncogene c-Myc by reducing its expression, not by altering cellular sensitivity to Myc. This finding impacts understanding of solid tumor development.

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Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Biology

Background:

  • c-Myc is a key oncogene implicated in various cancers, including solid tumors.
  • Disruption of tissue architecture is a hallmark of cancer, prompting investigation into cell organization's role in oncogene function.
  • Previous studies suggested that cellular organization confers resistance to Myc overexpression.

Purpose of the Study:

  • To investigate how epithelial cell organization affects oncogene c-Myc activity and expression.
  • To determine if cellular organization alters inherent cell sensitivity to Myc or modulates Myc expression levels.
  • To elucidate the mechanisms underlying Myc's role in solid tumor development within organized tissues.

Main Methods:

  • Studied mammary epithelial cells undergoing morphogenesis into 3D acinar structures in vitro.
  • Analyzed gene expression patterns during acinar formation.
  • Assessed Myc expression and transgene activity in both organized (acini) and unorganized cells.
  • Utilized retroviral and adenoviral vectors for Myc overexpression studies.

Main Results:

  • Epithelial cell organization into acini significantly decreases endogenous Myc expression.
  • Transcription from retroviral vectors is reduced during morphogenesis.
  • Myc transgene expression is substantially lower in organized acini compared to unorganized cells.
  • Forced Myc expression (adenovirus-mediated) in organized acini potently induces apoptosis, indicating preserved cellular sensitivity.

Conclusions:

  • Cellular organization suppresses Myc activity primarily by modulating its expression, not by altering intrinsic cellular responsiveness.
  • The apparent insensitivity of organized cells to Myc is due to decreased Myc expression, not resistance.
  • These findings suggest that other tumor suppression mechanisms, beyond tissue structure, counteract Myc in solid tumor progression.