Shotgun lipidomics on high resolution mass spectrometers
Related Experiment Video
Updated: Jun 1, 2026

Shotgun Lipidomics of Rodent Tissues
Published on: November 18, 2022
Dominik Schwudke1, Kai Schuhmann, Ronny Herzog
1MPI of Molecular Cell Biology and Genetics, 01307 Dresden, Germany.
Abstract:
Despite their compositional complexity, lipidomes comprise a large number of isobaric species that cannot be distinguished by conventional low resolution mass spectrometry and therefore in-depth MS/MS analysis was required for their accurate quantification. Here we argue that the progress in high resolution mass spectrometry is changing the concept of lipidome characterization. Because exact masses of isobaric species belonging to different lipid classes are not necessarily identical, they can now be distinguished and directly quantified in total lipid extracts. By streamlining and simplifying the molecular characterization of lipidomes, high resolution mass spectrometry has developed into a generic tool for cell biology and molecular medicine.
Related Concept Videos
Tandem Mass Spectrometry
High-Resolution Mass Spectrometry (HRMS)
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
MALDI-TOF Mass Spectrometry
Mass Spectrum: Interpretation

