Related Experiment Video
Updated: Jun 1, 2026

Modification and Functionalization of the Guanidine Group by Tailor-made Precursors
Published on: April 27, 2017
General base-guanidinium cooperation in bifunctional artificial phosphodiesterases
Riccardo Salvio1, Roberta Cacciapaglia, Luigi Mandolini
1Dipartimento di Chimica and IMC-CNR Sezione Meccanismi di Reazione, Università La Sapienza, P.le Aldo Moro 5, 00185 Roma, Italy.
Abstract:
Artificial phosphodiesterases that combine a guanidinium unit with a general base connected by a m-xylylene linker catalyze the transesterification of the RNA model compound 2-hydroxypropyl p-nitrophenyl phosphate (HPNP). The bifunctional catalysts presented in this work show varying extents of cooperation between catalytic units and a rate enhancement of 4 × 10(4) in the most favorable case.
Related Concept Videos
Phosphodiester Linkages
Phosphodiester bond forms when a phosphoric acid molecule (H3PO4) links with two hydroxyl groups (–OH) of two other molecules, forming two ester bonds. Two water molecules are released in this process. The phosphodiester bond is commonly found in nucleic acids (DNA and RNA) and plays a critical role in their structure and function.
Phosphodiester Bonds Link Nucleotides Together
DNA and RNA are polynucleotides or long chains of nucleotides that are linked together. A nucleotide is...
GTPases and their Regulation
Large G-proteins, also known...
Activation and Inactivation of G Proteins
DNA Base Pairing
Transducer Mechanism: Enzyme-Linked Receptors
Major types that are helpful drug targets include:

