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Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Neutron Spin Echo Spectroscopy as a Unique Probe for Lipid Membrane Dynamics and Membrane-Protein Interactions

Published on: May 27, 2021

Interaction between lipids and antimicrobial oligomers studied by solid-state NMR.

Weiguo Hu¹, Abhigyan Som, Gregory N Tew

¹Department of Polymer Science and Engineering, University of Massachusetts Amherst , Amherst, Massachusetts 01003, United States. whu@data.pse.umass.edu

The Journal of Physical Chemistry. B

|May 27, 2011

Summary

Synthetic antimicrobial oligomers interact with cell membranes. Solid-state NMR revealed these oligomers reside in lipid head groups, influencing membrane phase behavior and function.

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Area of Science:

Membrane biophysics
Lipid self-assembly
Antimicrobial agent interactions

Background:

Cell membranes possess complex lipid distributions influencing their function.
Antimicrobial peptides and analogues interact with cell membranes.

Purpose of the Study:

To investigate the phase behavior of mixed lipids (DOPE/DOPG) and their interaction with synthetic antimicrobial oligomers (AMOs).
To elucidate the structural basis of AMO-membrane interactions using solid-state NMR.

Main Methods:

Solid-state nuclear magnetic resonance (NMR) spectroscopy.
Construction of a lipid phase diagram across a range of water content.
Nuclear Overhauser spectroscopy (NOESY) to determine AMO localization.

Main Results:

A detailed phase diagram for DOPE/DOPG lipids was constructed, showing extensive multi-phase coexistence regions.
Lipid phase fractions are sensitive to sample preparation and thermal history, suggesting dynamic membrane structures.
AMOs were found to localize primarily in the lipid head group region.
The lipid DOPE did not show selective enrichment in the inverted hexagonal phase.

Conclusions:

The complex and dynamic phase behavior of lipid membranes, influenced by preparation and history, may underpin various membrane functions.
AMOs interact with the membrane surface, primarily within the head group region.
Understanding these interactions is crucial for designing effective antimicrobial strategies.